Due to questions raised over the Spiderman 3 poster, I decided to look into it some more.

The most significant alteration to Spider-Man's costume came about in the mid-1980s, after his return from the Secret Wars. He appeared in an almost all-black costume, with a large white black widow spider emblem on the chest and back, and with built-in webshooters on the back of his hands. The costume turned out to be a living symbiotic creature, capable of generating its own webbing and improving most of Spider-Man's abilities. Spider-Man rejected the symbiote after finding out it was alive and trying to merge with him. He did however wear a non-living version of the black costume until the new occupant of the living costume, Venom, frightened Mary Jane so badly that she could no longer stand to see Peter in the non-living black costume. Despite Mary Jane's objections, Peter has continued to use the black costume on a few occasions, when stealth was required (e.g. Peter Parker: Spider-Man #13).

^{From Wikipedia}